Catalogue The Microscop and Its Application
| Detail |
| | Name: | The Microscope and Its Application | | | | Publisher: | Leitz Wetzlar | | | | Classification: | Catalogue | | | | Category: | 512-69b | | | | Instrument Class: | valid for standard microscopes | | | | Instrument Type: | --- | | | | Title: | Microscopes | | | | Date: | I/74 | | | | Pages: | 109 | | | | Language: | English | |
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Content:
I Theory
The 2-stage image formation of the microscope .
First stage of magnification, second stage of magnification, involved optical path, Köhler's principle of illumination.
Microscopic image formation according to the principle of wave optics . . . . . . . . . . . . .
Resolution according to the principle of wave optics Aperture. . . . . . . . . . . . . . . .
Useful magnification with visual observation .
Useful magnificatian in phatamicrography . . . .
Darkground micrascopy . . . . . . . . . . .
Phase cantrast microscopy . . . . . . . . . .
Amplitude abjects, phase objects in brightfield, phase objects in phase cantrast, the phasecantrast microscape . . . . . . . . . . . . . . . .
lnterference cantrast microscapy, basic principle and functian . . . . . . . . . .
Refraction and dispersian of ligh
II Application
General hints . . . . . . . . . . . . . . .
Microscopy in brightfield . . . . . . . . . . .
Structure and function of the illuminating system
Use of the aperture diaphragm, the field diaphragm, framing and focusing the specimen . . . . . . .
Adjustment of the binocular tube Sand the binocular photo tube FSA. . . . . . . . . . . . . . .
Centring the lamp . . . . . . . . . . . . . .
Condenser adjustment . . . . . . . . . . . .
Change-over to higher magnification, correct objective/ eyepiece combination . . . . .
The correct coverglass for dry systems . . . . .
Dry objectives without correction mount . . . . .
Dry objectives with correction mount
Working with highest magnifications - immersion objectives . . . . . . . . . . . . . . . . .
Working with low magnifications . . . . . . . .
Microscopy in darkground . . . . . . . . . .
Accessories for darkground microscopy, some requirements
the microscope slide, specimen, coverglass, illumination has to meet . . . . . . . . .
Microscopy in phase contrast . . . . . . . . .
Effect of the thickness of the object, halo effect, influence of the embedding medium
Unear measurements in the microscope . . . .
A few practical hints
Photomicrography . . . . . . . . . . . . .
Microscope, illumination, and optical system. Which
camera format?
Films, exposure time, and filters .
I Black and white . . . . . . . . . . . . . .
II Colour .... . . . . . .. .. .... .
The photomicrograph . . . . . . . . . . . .
Specimens, setting up the illumination, framing and
focusing the microscopic image, use of the auxiliary
telescope, depth of field, determination of the
magnification, lamps for photomicrography
The most frequent faults in microscopy
III The instruments
The range of LEITZ stands . . . . . . . . . 69
Construction of a transmitted-light microscope . 70
Microscope tubes . . . . . . . . . . . . . 72
Tubelenssystems and revolving nosepieces. . 74
Objectstages. . . . . . . . . . . . . . . 75
Function of a planetary gear, functions of the single-knob adjustment . . . 78
llluminating devices . . . . . . 80
1 Lamp attachments
2a Built-in illuminators
2b Independent lamp housings
3 Lamps on pillar stands . . . 84
Optical system of the microscope
Objectives, eyepieces, microscope slides, coverglasses. . . . . . . . . . 86
Condensers . . . . . . . . 90
Filters . . . . . . . . . . . . . . . . . . . 92
Devices for photomicrography . . . . . . . . . 94
ORTHOMAT(il W, large-format camera, exposure meter
Devices for tracing, discussion, and projection. . . 98
Special accessories and other microscopes. . . . 101
MPV 2 microscope photometer, LEITZ T.A.S. Texture Analysis System, DIAVERT inverted microscope,
TS largefield stereomicroscope
Comparison of filters . 1 05
Index . . . . . . . . . . . . . . . . . . 106
